Journal article
N. Tyagi, Natia Qipshidze, C. Munjal, N. Metreveli, A. Dankowski, P. Mishra, U. Sen, D. Lominadze, S. Givvimani, S. Tyagi
2011
2011
Semantic Scholar
DOI
Cite
Cite
APA
Click to copy
Tyagi, N., Qipshidze, N., Munjal, C., Metreveli, N., Dankowski, A., Mishra, P., … Tyagi, S. (2011). Hydrogen Sulfide ameliorates mitochondrial MMP‐9 mediated mitochondria remodeling in cerebral ischemia.
Chicago/Turabian
Click to copy
Tyagi, N., Natia Qipshidze, C. Munjal, N. Metreveli, A. Dankowski, P. Mishra, U. Sen, D. Lominadze, S. Givvimani, and S. Tyagi. “Hydrogen Sulfide Ameliorates Mitochondrial MMP‐9 Mediated Mitochondria Remodeling in Cerebral Ischemia” (2011).
MLA
Click to copy
Tyagi, N., et al. Hydrogen Sulfide Ameliorates Mitochondrial MMP‐9 Mediated Mitochondria Remodeling in Cerebral Ischemia. 2011.
BibTeX Click to copy
@article{n2011a,
title = {Hydrogen Sulfide ameliorates mitochondrial MMP‐9 mediated mitochondria remodeling in cerebral ischemia},
year = {2011},
author = {Tyagi, N. and Qipshidze, Natia and Munjal, C. and Metreveli, N. and Dankowski, A. and Mishra, P. and Sen, U. and Lominadze, D. and Givvimani, S. and Tyagi, S.}
}
Abstract
Mitophagy is strongly implicated in stroke. Hydrogen Sulfide (H2S), an endogenous gaseous molecule, with antioxidant activities. Therefore objective of this study was to determine whether H2S ameliorates mitophagy in cerebral ischemia (CI) by reducing matrix metalloproteinase‐9 (MMP‐9). There weresix groups of mice: WT, WT/CI, WT/CI+ H2S, MMP‐9 knockout(KO), MMP‐9KO/CI and MMP‐9KO/CI++ H2S. CI was performed for45 mins. After 2hours of injury, mice were injected with NaHS (30μM/L, H2S donor) for 1week. The infarct area was measured using TTC staining. Permeability was determined by brain edema and Evans Blue extravasation. PCR and Western blot were used todetermine the expression of MMP‐9, TIMPs, mitochondrial redox stress and LC3‐I/II. Mitochondrial respiratory capacity and mitochondrial permeability transition (MPT) were measured by fluorescence‐dye. The result suggested Brain edema and Evans Blue leakage were reduced after cerebral ischemia in H2S treated group as compared to control group along with reduced brain infarct size. Western blot analysis indicated that H2S decreased mitochondrial oxidativedamage and ameliorated the MMP‐9 activation and loss of MPT, in part by increasing the protein and mRNA levels of TIMP‐1 and decreasing TIMP‐2 as compared to control. This study suggests a therapeutic role of H2S in Cerebral Ischemia.
