Journal article
C. Munjal, J. Falcone, Natia Qipshidze, P. Mishra, Suresh Tyagi
2010
2010
Semantic Scholar
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APA
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Munjal, C., Falcone, J., Qipshidze, N., Mishra, P., & Tyagi, S. (2010). DDAH‐2 & eNOS in Mesenteric Vascular Remodeling : Role of Fenugreek.
Chicago/Turabian
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Munjal, C., J. Falcone, Natia Qipshidze, P. Mishra, and Suresh Tyagi. “DDAH‐2 &Amp; ENOS in Mesenteric Vascular Remodeling : Role of Fenugreek” (2010).
MLA
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Munjal, C., et al. DDAH‐2 &Amp; ENOS in Mesenteric Vascular Remodeling : Role of Fenugreek. 2010.
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@article{c2010a,
title = {DDAH‐2 & eNOS in Mesenteric Vascular Remodeling : Role of Fenugreek},
year = {2010},
author = {Munjal, C. and Falcone, J. and Qipshidze, Natia and Mishra, P. and Tyagi, Suresh}
}
Abstract
Although mesenteric vessels played a crucial role in vascular remodeling, the mechanism was unclear. A critical factor, for the development of hypertension was increase in resistance in splanchnic blood flow by endothelial dysfunction. Increased homocysteine (Hcy) levels known as hyperhomocysteinimia(HHcy), causing vascular dysfunction. DDAH degrades ADMA, an endogenous inhibitor of nitric oxide synthetas (eNOS). Matrix metalloprotease (MMP) caused an increase in collagen/elastin ratio. Fenugreek (FG) primarily used as antidiabetic, also reported to have restoration of endothelial function. We hypothesized Hcy decreased mesentery blood flow in part by imbalancing the MMP/TIMP axis and decreasing the eNOS/DDAH activity. The treatment with FG mitigated blood flow and hypertension. To test this hypothesis we used WT(b) WT+ FG (c) WT+High Meth diet (d)WT+ High Meth diet+ FG, i.p. injection. The protein expression of MMPs, TIMPs and redox proteins were measured by Western blot. MMP‐2, 9 activities was measured by zymography. Tissue sections were used for to study elastin/collagen ratio. The data suggested Hcy decreased blood flow and matrix disruption by decreasing DDAH‐2, eNOS expression, FG ameliorated it. Therefore we concluded that FG migrate, decreased mesentery blood flow, MMP/TIMP axis distruption, decreasing the eNOS/DDAH activity by Hcy.
